1. Deeply cough 3 -5 times
2. Fit the funnel onto the tube and spit oral fluid
3. Remove the funnel, the oral fluid (non - bubble) should just reach the scale line
4. Place the tube into the tube holder, add entire disposable buffer.
5. Squeeze the tube 10 - 15 times.
6. Invert the tube and add 2 drops of solution to the specimen well(s) of the test device and then start the timer.
7. Read the result at 15 minutes.
8. After test is completed, place the all the components of the test kit in plastic Biosafety Bag provided and dispose it into trash.